Circulating bone marrow (BM)–derived endothelial progenitor cells (EPCs) are recruited to the site of tissue regeneration and substantially contribute to neovascularization and re-endothelialization after acute vascular injury. Intravenous infusion of EPCs after ischemia was shown to improve neovascularization and cardiac function Conversely, BM-derived EPCs participate in the pathogenesis of various diseases, such as cancer, retinopathy, and atherosclerosis. Impaired recruitment of BM-derived endothelial and hematopoietic precursor cells is known to block both tumor angiogenesis And growth. BM-derived progenitors also contribute to the formation of the microvasculature in allograft arteriosclerotic lesions., EPCs have both physiologic and pathologic roles; thus, they have been widely considered for establishing New therapeutic strategies for the treatmen The number of circulating EPCs may limit the ultimate magnitude of angiogenesis therapies and, therefore, strategies that are based on the administration of ex vivo–expanded populations of EPCs harvested from the patient's circulating blood appear promising.
1. Human umbilical cord blood (HUCB) samples (∼50 mL each) were collected from fresh placentas with attached umbilical cords by gravity flow.
2. EPCs were isolated by density gradient centrifugation over Biocoll (Biochrom, Berlin, Germany) for 30 minutes at 400g and washed 3 times in PBS (Biochrom).
3. One million cells were seeded onto 6-well plates coated with human fibronectin in Primary endothelial basal medium.
4. The medium was supplemented with endothelial growth medium containing fetal bovine serum, human VEGF-A, human fibroblast growth factor-B, human epidermal growth factor, insulin-like growth factor 1 (IGF1), and ascorbic acid in appropriate amounts.
5. After 3 days, non-adherent cells were removed, and fresh culture medium was applied.
6. Cultures were maintained for 7 days.
7. Phenotypic analyses of the cells were performed on days 3, 5, and 7.
8. EPC identification and estimation of culture purity (90%-95%) were determined by staining cells with FITC-labeled Ulex europaeus lectin I and uptake of Dil-conjugated acetylated low-density lipoprotein.
References
1. Asahara T, Murohara T, Sullivan A, et al. Isolation of putative progenitor endothelial cells for angiogenesis. Science 1997;275:964-967.
2. Sata M. Role of circulating vascular progenitors in angiogenesis, vascular healing, and pulmonary hypertension: lessons from animal models. Arterioscler Thromb Vasc Biol 2006;26:1008-1014.
3. Orimo A, Gupta PB, Sgroi DC, et al. Stromal fibroblasts present in invasive human breast carcinomas promote tumor growth and angiogenesis through elevated SDF-1/CXCL12 secretion. Cell 2005;121:335-348.
4. Lyden D, Hattori K, Dias S, et al. Impaired recruitment of bone-marrow-derived endothelial and hematopoietic precursor cells blocks tumor angiogenesis and growth. Nat Med 2001;7:1194-1201.
5. Otani A, Kinder K, Ewalt K, Otero FJ, Schimmel P, Friedlander M. Bone marrow-derived stem cells target retinal astrocytes and can promote or inhibit retinal angiogenesis. Nat Med 2002;8:1004-1010.
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PRODUCT | SPECIFICATION | NORMAL PACKING |
Analgin /Diyron /Metamizol |
Injection 0.5mg/2ml 2.5g/5ml Tablet 0.5g |
100amps/Box 1000's/Tin |
Celecoxib | Capsule 200mg | 10x10`s/Box |
Diclofenac Sodium | Inj. 75mg/3Ml | 5's/Tray/Box |
Ibuprofene |
Tablet 0.2g 0.4g Syrup 100mg/5ml 25ml |
1000's/Tin 1`s/Box |
Indomethacin | Capsule 25mg | 1000's/Tin |
Paracetamol |
Tablet 0.5g 0.3g Injection 300mg/2ml Infusion 1g/100ml |
1000's/Box 10ampoules/Box 1`s/Bottle |
Piroxicam |
Capsule 20mg ; 40mg Injection 20mg/2ml |
10`Sx10/Box 100`S/Box |
Dexamethason Phosphate | Inj. 4mg/Ml | 5x5amps/Tray/Box |
Hydrocortisone Sodium Succinate | Powder Injection 100mg | 50vials/Box |
Levonorgestrol | Tablet 0.75mg | 10`s x10/Box |
Levonorgestrel +Ethinylestradiol | Tablet 0.15mg+0.03mg | 21`s/Blister |
Prednisolone | Tablet 5mg | 1000's/Tin |
Progesterone | Oil Inj. 20mg/Ml ,25mg/Ml | 100amps/Box |
Testosterone Proplonate | Oil Inj.10mg/Ml 25mg/Ml | 10'sx10tray/Box |
Triamcinolone acetonide | Inj. 80mg/2ml | 10`s/Box |
Adrenine | Injection 1mg/Ml | 10`s/Box |
Atracurium Besylate | Powder for inj. 25mg/2.5ml; 50mg/5ml | 1`s/Box |
Atropine | Inj. 1mg/Ml | 10'sx10tray/Box |
Chlopromazine Hcl | Injection 25mg/2ml | 100`S/Box |
Diazepam Inj. | Inj. 20mg/2Ml | 10`S/Box |
Promethazine | Inj. 25mg/2ml | 10'sx10tray/Box |
Ambroxol HCL | syrup 30mg/5ml 100mL | 1Bottle/Box |
Aminophilline | Injection 0.25g 10ml | 50's/Box |
Salbutamol | Aerosol 100mcg/Dose 200Doses | 1bottle/Box |
Beclomethasone Dipropionate | Aerosol 100mcg 200doses | 1`s/Box |
Xylometazoline | Nasal drop 0.1% 10Ml | Plastic Vial |
Diosmin Tablet | Tablet 500mg | 10`sx10/Box |
Metformini Hcl | Tablet 0.25g | 1000's/Tin |
Orlistat | Tablet 120mg | 10x10`s/Box |
Vecuronium Bromide | Powder for Injection 4mg | 1Vial/Box |
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