High Performance Liquid Chromatography (HPLC) is an effective and reliable technology that has become an important tool for many laboratory studies. One of the most common problems with HPLC is the effect of contaminants in the solvent on the analytical results.
Particulates can damage the plunger rod and seal and the injection valve. Particulate matter can also block the column and melt it, which can result in increased system pressure. Particulate matter can also behave as another solid phase, possibly altering the composition of the sample.
Organic matter can affect the resolution and integration of chromatographic peaks, and can also lead to ghost peaks, possibly changing the selectivity of the stationary phase and affecting the chromatographic baseline.
Changes in ion concentration can affect the separation results, and some of the ions that absorb UV will have an effect on the peak. Certain corrosive ions also reduce the life of accessories such as high pressure infusion pumps.
The colloid will be irreversibly adsorbed on the stationary phase, affecting the separation of the column.
Microorganisms can clog the column, and its metabolites increase pollutants such as organic matter and particulate matter.
Bottled purified water and distilled water are the pure water used in some HPLC analysis. Since the bottled purified water and distilled water are only ordinary pure water instead of ultrapure water, the water still contains a small amount of impurities such as ions and organic substances to prepare liquid phase and wash. Delimination can not only contaminate expensive columns, but also affect HPLC out of a flat baseline.
The purification process of bottled purified water usually includes adsorption, filtration, reverse osmosis and the like. For the removal of particulate matter and organic matter, the removal of trace ions and organic matter cannot meet the requirements of high-sensitivity HPLC experiments. Moreover, since the packaging is usually mostly PVC bottles, the oxygen permeability is high and there is a certain chemistry. The amount of dissolution, which contaminates the pure water in the bottle, especially after a period of storage, the water quality drops more, so it appears on the HPLC chromatogram, although there is no specific absorption peak, but there is a serious interference at the baseline.
Distilled water refers to pure water prepared by distillation. The water can be distilled once and several times. After one-time distillation of water, the non-volatile components remain in the vessel and the volatile components enter the initial fraction of distilled water. Usually only the middle portion of the fraction is collected, accounting for about 60%. To obtain purer water, An alkaline potassium permanganate solution is added to the distilled water to remove organic matter and carbon dioxide; and a non-volatile acid (sulfuric acid or phosphoric acid) is added to make the ammonia a non-volatile ammonium salt. By HPLC detection of double distilled water, it was found that 254 nm and 214 nm showed strong absorption peaks at 22-27 minutes, which indicated that there was a strong hydrophobic organic matter contamination, which was caused by azeotropy in the distillation process. Some volatile organic compounds are not completely removed.
The UPA , UPB , UPC and UPD series of ultra-pure water machines launched by Shanghai ç»¼åˆ combine various reverse purification processes such as reverse osmosis, ion exchange, activated carbon adsorption, membrane filtration, ultrafiltration and UV oxidation. The water conductivity reaches 18.2MΩ. •cm, the water quality exceeds the national standard water standard and is stable and measurable. The ultra-pure water is ready to use, no pollution is introduced due to storage, and the water quality is guaranteed. It is more suitable for users than using bottled purified water or distilled water. The need for precision instrumental analysis.
Laundry Detergent,Organic Detergent Liquid,Laundry Liquid Detergent,Detergent Liquid
Wuxi Keni Daily Cosmetics Co.,Ltd , https://www.wxkenidaily.com