Complete global genome sequencing of poplars for the first time in the world

It was learned from Nanjing Forestry University that the entire genome sequencing project of poplar initiated and implemented by the US Department of Energy has been successfully completed, and the full sequence database was opened to the public on September 21 this year. Associate Professor Yin Xiaoming, a research scientist at Nanjing Forestry University, participated in the study. Press releases and celebrations of the poplar genome are scheduled for December 6 in California. The study is expected to greatly shorten the period of species improvement of this important species of poplar trees, which has spanned the millennia barrier for decades. The completion of the study made poplars the third full-sequence plant after Arabidopsis and rice, and was the first perennial woody plant to determine the whole genome sequence. Poplar is thus widely accepted as a model species for the study of perennial plant genomes, which makes the work of great scientific significance. Poplar is also an important species of industrial timber. The implementation of poplar genome project will provide knowledge reserves for the development of bio-energy, and has important practical value. At present, the improvement of poplars is still in a semi-wild primary improvement stage. On the basis of genome research, useful alleles are developed between different tree species of poplar by means of population and quantitative genetics, and through genetic engineering. The means to carry out genetic recombination is expected to complete the improvement process of general crops for several thousand years in several decades. The entire poplar genome was sequenced using a "shotgun assay." A total of 7,649,993 sequence fragments were included in the sequence library to remove chloroplast genome contamination. The sequence was approximately 8 genome lengths. At present, the assembly of sequence splicing has completed 483Mb, accounting for more than 90% of the total length of poplar genomic physics, basically covering most of the poplar genomic autosomes. Based on gene chip and single-nucleotide polymorphism detection techniques, the work on the filling of small sequences and the filling of sequence gaps is in progress. It is expected that this part of the work will be completed next year. Associate Professor Yin Xiaoming from Nanjing Forestry University has been involved in this research since 2001. The annotation of the poplar genome will be completed in early December of this year. Mr. Jerry, the head of the International Poplar Genome Project Collaboration Group, believes that poplars account for the largest share of forestry production in China, and therefore China’s future in the application of poplar genome information. The research may be in the front ranks of the world. The completion of the poplar genome project provided unprecedented opportunities and challenges for scientists engaged in forestry and biotechnology in China.

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