In Ganoderma lucidum cultivation, it often causes different degrees of damage due to the contamination of various bacteria, among which Trichoderma viride is the most frequent and most harmful, and can occur in all stages of Ganoderma lucidum cultivation. Trichoderma viride is widely found in various organic materials and soils in nature. It also often floats in the air in the form of conidia. It has strong adaptability, especially on nutrient-rich substrates, and it spreads quickly. It can compete with the cultivated Ganoderma lucidum to feed nutrients and consume nutrients. It can also secrete toxins to destroy the cytoplasm of Ganoderma lucidum mycelium and inhibit the growth of Ganoderma lucidum mycelium. In the cultivation, good results can be obtained if preventive measures are mainly adopted and supplemental treatment is taken. First, choose a variety of Ganoderma lucidum that has good resistance to miscellaneous and strong hypha growth. Second, strictly select cultivation species. The selected strains require pure seed, strong mycelium viability, white, thick, robust, and appropriate bacterial age. Third, do a good job in the cleanliness of the cultivation environment. To remove the mushroom residues and garbage in the mushroom house, thoroughly clean the cultivation racks, and conduct space disinfection to eliminate the hidden places of the bacteria to reduce the transmission medium. Doing a good job in environmental sanitation can have a multiplier effect in preventing pollution. Fourth, strict choice of materials. Cultivation materials require fresh, no mildew, before exposure to a few days before use, culture material formula should be reasonable, the main material and auxiliary materials should be fully mixed, water content controlled at 60% to 70%, the amount of appropriate, moderate elasticity, installed Immediately after high-pressure or atmospheric pressure sterilization, to prevent acidification of the medium. Sterilization should be thorough. Fifth, to ensure no contamination of bacteria when inoculated. During the inoculation, the operator's hands, clothes, and inoculation tools and materials used shall be strictly disinfected. The inoculation shall be as quick and proficient as possible to prevent the introduction of germs during the inoculation. Damaged bags during sterilization should be sealed with tape and disinfected with 75% alcohol at the closure. 6. Increase the inoculation amount. Appropriately increasing the inoculum size will enable Ganoderma lucidum to rapidly occupy the site with its absolute advantage, reduce the contamination of the bacteria, and act as a mushroom inhibitor. 7. Ensure that the cultivation room has a suitable microclimate and train good mycelium. Keep the temperature around 25°C, 60%-70% humidity, pay attention to ventilation, prevent high temperature and humidity, create the most suitable environmental conditions for the growth of Ganoderma lucidum mycelia, and promote the rapid growth of Ganoderma mycelium to occupy the entire material surface rapidly. VIII. Grasp the management of the Shiba stage. After the original Ganoderma lucidum grows, it is necessary to unplug the tampon or open the bag in time to avoid damage to the original base and to infect the green Trichoderma. Do a good job of heat preservation and moisturizing work. At the same time, strengthen ventilation, and give a certain amount of light to promote the healthy growth of the original base into fruiting bodies. Nine, strengthen early prevention and control. During the growth phase of mycelium, if the material surface is found to be contaminated with green Trichoderma, the affected area can be scrubbed with lime water, or blocked with lime slurry, or injected with 5% lime acid solution; if the fruiting body is infected with green Trichoderma, it should be removed in time to prevent spread. The use of carbendazim and harvested pure 400 to 1500 times liquid spices, the inhibitory effect on Trichoderma viride is very good, but also inhibit the growth of Ganoderma mycelium. The 0.35 g/L copper hydroxide mix can effectively inhibit the growth of Trichoderma viride, while having no effect on the growth of Ganoderma lucidum mycelium.
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